Composite

Part:BBa_K090402:Design

Designed by: Daniel Goodman   Group: iGEM08_Cambridge   (2008-10-29)


B. subtilis Episomal Vector with Constitutive GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5187
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5193
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5187
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5187
    Illegal XbaI site found at 5202
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI.rc site found at 4261
    Illegal BsaI.rc site found at 6124
    Illegal SapI site found at 3178


Design Notes

To build this part, we used 4 different linear DNA pieces assembled with 15 base pair homologous regions PCRed to the ends. These 4 different pieces were then assembled via the InFusion assembly method by Clontech.


Source

See data for BBa_K90401. This part is the same, but with the pUpp promoter, a gram-positive RBS, and a GFP with terminator.

References